Acetyl-Histone H4-K12 pAb

ProSci
Product Code: PSI-15-437
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-437-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 6
Immunohistochemistry of paraffin-embedded human appendix using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
2 / 6
Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
3 / 6
Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
4 / 6
Immunohistochemistry of paraffin-embedded mouse spinal cord using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
5 / 6
Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
6 / 6
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded human appendix using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 antibody (15-437) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic acetylated peptide around K12 of human Histone H4 (NP_001029249.1).
Modifications:
Acetylated
NCBI Gene ID #:
8366
NCBI Official Name:
histone cluster 1, H4a
NCBI Official Symbol:
H4K12ac
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P62805
User NOte:
Optimal dilutions for each application to be determined by the researcher.