ATP2A3 Antibody

ProSci
Product Code: PSI-15-440
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-440-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using ATP2A3 antibody (15-440) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human breast cancer using ATP2A3 antibody (15-440) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human lung using ATP2A3 antibody (15-440) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human uterine cancer using ATP2A3 antibody (15-440) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ATP2A3 antibody (15-440) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human breast cancer using ATP2A3 antibody (15-440) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using ATP2A3 antibody (15-440) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using ATP2A3 antibody (15-440) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
ATP2A3, SERCA3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 360-600 of human ATP2A3 (NP_005164.2).
NCBI Gene ID #:
489
NCBI Official Name:
ATPase, Ca++ transporting, ubiquitous
NCBI Official Symbol:
ATP2A3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 114kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
Q93084
User NOte:
Optimal dilutions for each application to be determined by the researcher.