VPS26A Antibody

Contact us
ProSci
Product Code: PSI-15-456
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-456-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using VPS26A antibody (15-456) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 2
Immunofluorescence analysis of NIH-3T3 cells using VPS26A Polyclonal Antibody (15-456) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using VPS26A antibody (15-456) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of NIH-3T3 cells using VPS26A Polyclonal Antibody (15-456) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
VPS26A, vacuolar protein sorting 26 homologue A (yeast), FLJ12930, HB58, Hbeta58, VPS26, Hbeta58 homolog, vacuolar protein sorting 26 (yeast homolog), vacuolar protein sorting 26 (yeast), vacuolar protein sorting 26 homolog A
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 208-327 of human VPS26A (NP_004887.2).
NCBI Gene ID #:
9559
NCBI Official Name:
vacuolar protein sorting 26 homolog A (S. pombe)
NCBI Official Symbol:
VPS26A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O75436
User NOte:
Optimal dilutions for each application to be determined by the researcher.