PRKAG2 Antibody

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ProSci
Product Code: PSI-15-465
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-465-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PRKAG2 antibody (15-465) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat liver using PRKAG2 antibody (15-465) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat ovary using PRKAG2 antibody (15-465) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using PRKAG2 antibody (15-465) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat liver using PRKAG2 antibody (15-465) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using PRKAG2 antibody (15-465) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
AAKG, AAKG2, CMH6, H91620p, WPWS, 5'-AMP-activated protein kinase subunit gamma-2, AMPK subunit gamma-2, epididymis secretory sperm binding protein, protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human PRKAG2 (NP_077747.1).
NCBI Gene ID #:
51422
NCBI Official Name:
protein kinase AMP-activated non-catalytic subunit gamma 2
NCBI Official Symbol:
PRKAG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 63kDa
Purification:
Affinity purification
Research Area:
Autophagy, Cell Cycle, Signal Transduction
Swissprot #:
Q9UGJ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.