PIGZ Antibody

ProSci
Product Code: PSI-15-533
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-533-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of U-87MG cells, using PIGZ antibody (15-533) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.

Western blot analysis of extracts of U-87MG cells, using PIGZ antibody (15-533) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.

Further Information

Additional Names:
GPI-MT-IV, PIG-Z, SMP3, GPI mannosyltransferase 4, GPI mannosyltransferase IV, SMP3 mannosyltransferase, dol-P-Man dependent GPI mannosyltransferase, phosphatidylinositol glycan, class Z, phosphatidylinositol-glycan biosynthesis class Z protein
Application Note:
WB: 1:500 - 1:2000
Background:
The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells that serves to anchor proteins to the cell surface. This gene encodes a protein that is localized to the endoplasmic reticulum, and is involved in GPI anchor biosynthesis. As shown for the yeast homolog, which is a member of a family of dolichol-phosphate-mannose (Dol-P-Man)-dependent mannosyltransferases, this protein can also add a side-branching fourth mannose to GPI precursors during the assembly of GPI anchors.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 435-579 of human PIGZ (NP_079439.2).
NCBI Gene ID #:
80235
NCBI Official Name:
phosphatidylinositol glycan anchor biosynthesis class Z
NCBI Official Symbol:
PIGZ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 63kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q86VD9
User NOte:
Optimal dilutions for each application to be determined by the researcher.