CAMK1 Antibody

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ProSci
Product Code: PSI-15-570
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-15-570-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CAMK1 antibody (15-570) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human stomach using CAMK1 antibody (15-570) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using CAMK1 antibody (15-570) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using CAMK1 antibody (15-570) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spinal cord using CAMK1 antibody (15-570) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using CAMK1 antibody (15-570) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using CAMK1 antibody (15-570) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using CAMK1 antibody (15-570) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CAMK1 antibody (15-570) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CAMK1 antibody (15-570) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using CAMK1 antibody (15-570) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CAMK1 antibody (15-570) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM-KI, CaM kinase I alpha, CaMKI-alpha, CAMK1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human CAMK1 (NP_003647.1).
NCBI Gene ID #:
8536
NCBI Official Name:
Calcium/calmodulin-dependent protein kinase type 1
NCBI Official Symbol:
CAMK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 41kDa
Purification:
Affinity purification
Research Area:
Immunology, Neuroscience, Signal Transduction
Swissprot #:
Q14012
User NOte:
Optimal dilutions for each application to be determined by the researcher.