FLCN Antibody

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ProSci
Product Code: PSI-15-583
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-583-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using FLCN antibody (15-583) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.
2 / 4
Immunofluorescence analysis of C6 cells using FLCN antibody (15-583) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of NIH/3T3 cells using FLCN antibody (15-583) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of U2OS cells using FLCN antibody (15-583) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using FLCN antibody (15-583) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of C6 cells using FLCN antibody (15-583) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using FLCN antibody (15-583) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using FLCN antibody (15-583) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Folliculin, BHD skin lesion fibrofolliculoma protein, Birt-Hogg-Dube syndrome protein, FLCN, BHD
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene are associated with Birt-Hogg-Dube syndrome, which is characterized by fibrofolliculomas, renal tumors, lung cysts, and pneumothorax. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human FLCN (NP_653207.1).
NCBI Gene ID #:
201163
NCBI Official Name:
Folliculin
NCBI Official Symbol:
FLCN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q8NFG4
User NOte:
Optimal dilutions for each application to be determined by the researcher.