GCG Antibody

ProSci
Product Code: PSI-15-629
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-629-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GCG antibody (15-629) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded mouse pancreatic islet using GCG antibody (15-629) at dilution of 1:100 (40x lens).
3 / 4
Immunofluorescence analysis of rat pancreas using GCG antibody (15-629) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of mouse pancreas using GCG antibody (15-629) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GCG antibody (15-629) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse pancreatic islet using GCG antibody (15-629) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat pancreas using GCG antibody (15-629) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse pancreas using GCG antibody (15-629) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
GCG, GLP1, GLP2, GRPP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-180 of human GCG (NP_002045.1).
NCBI Gene ID #:
2641
NCBI Official Name:
glucagon
NCBI Official Symbol:
GCG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 21kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P01275
User NOte:
Optimal dilutions for each application to be determined by the researcher.