MYH13 Antibody

ProSci
Product Code: PSI-15-636
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-636-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MYH13 antibody (15-636) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunofluorescence analysis of rat eye using MYH13 Polyclonal Antibody (15-636) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse eye using MYH13 Polyclonal Antibody (15-636) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MYH13 antibody (15-636) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of rat eye using MYH13 Polyclonal Antibody (15-636) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse eye using MYH13 Polyclonal Antibody (15-636) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
MyHC-IIL, MyHC-eo, myosin-13, extraocular muscle myosin heavy chain, extraocular myosin heavy chain, myosin heavy chain, skeletal muscle, extraocular, myosin heavy chain, skeletal muscle, laryngeal, myosin, heavy chain 13, skeletal muscle, myosin, heavy polypeptide 13, skeletal muscle, superfast myosin
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1240-1320 of human MYH13 (NP_003793.2).
NCBI Gene ID #:
8735
NCBI Official Name:
myosin heavy chain 13
NCBI Official Symbol:
MYH13
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 250kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9UKX3
User NOte:
Optimal dilutions for each application to be determined by the researcher.