GLI1 Antibody

ProSci
Product Code: PSI-15-663
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-663-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 6
Western blot analysis of extracts of various cell lines, using GLI1 antibody (15-663) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 6
Immunohistochemistry of paraffin-embedded rat testis using GLI1 antibody (15-663) at dilution of 1:100 (40x lens).
3 / 6
Immunohistochemistry of paraffin-embedded human mammary cancer using GLI1 antibody (15-663) at dilution of 1:100 (40x lens).
4 / 6
Immunohistochemistry of paraffin-embedded mouse testis using GLI1 antibody (15-663) at dilution of 1:100 (40x lens).
5 / 6
Immunofluorescence analysis of HeLa cells using GLI1 antibody (15-663) at dilution of 1:100. Blue: DAPI for nuclear staining.
6 / 6
Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody (15-663) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using GLI1 antibody (15-663) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using GLI1 antibody (15-663) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using GLI1 antibody (15-663) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using GLI1 antibody (15-663) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using GLI1 antibody (15-663) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody (15-663) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
GLI1, GLI
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human GLI1 (NP_001153517.1).
NCBI Gene ID #:
2735
NCBI Official Name:
GLI family zinc finger 1
NCBI Official Symbol:
GLI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 130kDa/160kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience, Signal Transduction, Stem Cell, Transcription
Swissprot #:
P08151
User NOte:
Optimal dilutions for each application to be determined by the researcher.