CD36 Antibody

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ProSci
Product Code: PSI-15-682
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-15-682-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of THP-1 cells, using CD36 antibody (15-682) at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) at 37°C for overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 3
Western blot analysis of extracts of various cell lines, using CD36 antibody (15-682) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
3 / 3
Immunofluorescence analysis of THP-1 cells using CD36 antibody (15-682) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of THP-1 cells, using CD36 antibody (15-682) at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) at 37°C for overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Western blot analysis of extracts of various cell lines, using CD36 antibody (15-682) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunofluorescence analysis of THP-1 cells using CD36 antibody (15-682) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
CD36, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3, BDPLT10
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 301-400 of human CD36 (NP_001120916.1).
NCBI Gene ID #:
948
NCBI Official Name:
CD36 molecule (thrombospondin receptor)
NCBI Official Symbol:
CD36
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70-110kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Stem Cell
Swissprot #:
P16671
User NOte:
Optimal dilutions for each application to be determined by the researcher.