HCRTR1 Antibody

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ProSci
Product Code: PSI-15-697
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-697-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HCRTR1 antibody (15-697) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunofluorescence analysis of rat brain using HCRTR1 antibody (15-697) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using HCRTR1 antibody (15-697) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunofluorescence analysis of rat brain using HCRTR1 antibody (15-697) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
HCRTR1, OX1R, hypocretin (orexin) receptor 1, orexin receptor, orexin receptor 1, orexin receptor-1, hypocretin receptor 1, hypocretin receptor-1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2).
NCBI Gene ID #:
3061
NCBI Official Name:
hypocretin (orexin) receptor 1
NCBI Official Symbol:
HCRTR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Obesity, Signal Transduction
Swissprot #:
O43613
User NOte:
Optimal dilutions for each application to be determined by the researcher.