NDUFA5 Antibody, KO Validated

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ProSci
Product Code: PSI-15-706
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-15-706-50uL50uL£505.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts from normal (control) and NDUFA5 knockout (KO) HeLa cells, using NDUFA5 antibody (15-706) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded human tonsil using NDUFA5 antibody (15-706) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using NDUFA5 antibody (15-706) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using NDUFA5 antibody (15-706) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using NDUFA5 Polyclonal Antibody (15-706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts from normal (control) and NDUFA5 knockout (KO) HeLa cells, using NDUFA5 antibody (15-706) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human tonsil using NDUFA5 antibody (15-706) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NDUFA5 antibody (15-706) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using NDUFA5 antibody (15-706) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using NDUFA5 Polyclonal Antibody (15-706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
B13, CI-13KD-B, CI-13kB, NUFM, UQOR13, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa, NADH-ubiquinone oxidoreductase 13 kDa-B subunit, complex I 13kDa subunit B, complex I subunit B13, type I dehydrogenase, ubiquinone reductase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
NCBI Gene ID #:
4698
NCBI Official Name:
NADH:ubiquinone oxidoreductase subunit A5
NCBI Official Symbol:
NDUFA5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 13kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience
Swissprot #:
Q16718
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.