NFE2L1 Antibody

ProSci
Product Code: PSI-15-708
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-708-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NFE2L1 antibody (15-708) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat spleen using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human uterus using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U-2 OS cells using CCDC59 antibody (15-521) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NFE2L1 antibody (15-708) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat spleen using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NFE2L1 antibody (15-708) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using CCDC59 antibody (15-521) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
NFE2L1, FLJ00380, LCR-F1, NRF1, TCF11
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene, NFE2L1, and for 'nuclear respiratory factor 1' which has an official symbol of NRF1.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 515-772 of human NFE2L1 (NP_003195.1).
NCBI Gene ID #:
4779
NCBI Official Name:
nuclear factor, erythroid 2-like 1
NCBI Official Symbol:
NFE2L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 64kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Transcription
Swissprot #:
Q14494
User NOte:
Optimal dilutions for each application to be determined by the researcher.