ERLIN1 Antibody

ProSci
Product Code: PSI-15-759
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-759-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ERLIN1 antibody (15-759) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human appendix using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ERLIN1 antibody (15-759) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human appendix using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ERLIN1 antibody (15-759) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
ERLIN1, C10orf69, Erlin-1, KE04, KEO4, RP11-316M21.1, SPFH1, SPG62
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is part of a protein complex that mediates degradation of inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum. The encoded protein also binds cholesterol and regulates the SREBP signaling pathway, which promotes cellular cholesterol homeostasis. Defects in this gene have been associated with spastic paraplegia 62.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2).
NCBI Gene ID #:
10613
NCBI Official Name:
ER lipid raft associated 1
NCBI Official Symbol:
ERLIN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 39kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O75477
User NOte:
Optimal dilutions for each application to be determined by the researcher.