BRMS1 Antibody

ProSci
Product Code: PSI-15-766
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-766-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using BRMS1 antibody (15-766) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat liver using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using BRMS1 antibody (15-766) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using BRMS1 antibody (15-766) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Breast cancer metastasis-suppressor 1, BRMS1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human BRMS1 (NP_056214.1).
NCBI Gene ID #:
25855
NCBI Official Name:
Breast cancer metastasis-suppressor 1
NCBI Official Symbol:
BRMS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
Q9HCU9
User NOte:
Optimal dilutions for each application to be determined by the researcher.