AQP1 Antibody

ProSci
Product Code: PSI-15-840
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-840-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using AQP1 antibody (15-840) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 4
Immunohistochemistry of paraffin-embedded mouse kidney using AQP1 antibody (15-840) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat brain using AQP1 antibody (15-840) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of NIH/3T3 cells using AQP1 antibody (15-840) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using AQP1 antibody (15-840) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded mouse kidney using AQP1 antibody (15-840) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using AQP1 antibody (15-840) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using AQP1 antibody (15-840) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Aquaporin-1, AQP-1, Aquaporin-CHIP, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule, AQP1, CHIP28
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human AQP1 (NP_932766.1).
NCBI Gene ID #:
358
NCBI Official Name:
Aquaporin-1
NCBI Official Symbol:
AQP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P29972
User NOte:
Optimal dilutions for each application to be determined by the researcher.