CTRB1 Antibody

ProSci
Product Code: PSI-15-844
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-844-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CTRB1 antibody (15-844) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 5
Immunohistochemistry of paraffin-embedded mouse lung using CTRB1 antibody (15-844) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded mouse pancreas using CTRB1 antibody (15-844) at dilution of 1:100 (40x lens).
4 / 5
Immunofluorescence analysis of rat pancreas using CTRB1 Polyclonal Antibody (15-844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse pancreas using CTRB1 Polyclonal Antibody (15-844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CTRB1 antibody (15-844) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded mouse lung using CTRB1 antibody (15-844) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using CTRB1 antibody (15-844) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat pancreas using CTRB1 Polyclonal Antibody (15-844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse pancreas using CTRB1 Polyclonal Antibody (15-844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CTRB1, CTRB, FLJ42412, MGC88037
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a member of the serine protease family of enzymes and forms a principal precursor of the pancreatic proteolytic enzymes. The encoded preproprotein is synthesized in the acinar cells of the pancreas and secreted into the small intestine where it undergoes proteolytic activation to generate a functional enzyme. This gene is located adjacent to a related chymotrypsinogen gene. This gene encodes distinct isoforms, some or all of which may undergo similar processing to generate the mature protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 79-263 of human CTRB1 (NP_001897.4).
NCBI Gene ID #:
1504
NCBI Official Name:
chymotrypsinogen B1
NCBI Official Symbol:
CTRB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P17538
User NOte:
Optimal dilutions for each application to be determined by the researcher.