KRT14 Antibody

ProSci
Product Code: PSI-15-857
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-857-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using KRT14 antibody (15-857) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded Rat lung using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Human esophagus using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Mouse kidney using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of Rat skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of Human skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of Mouse skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using KRT14 antibody (15-857) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded Rat lung using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human esophagus using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Rat skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Human skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Mouse skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
KRT14, K14, NFJ, CK14, EBS3, EBS4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human KRT14 (NP_000517.2).
NCBI Gene ID #:
3861
NCBI Official Name:
keratin 14
NCBI Official Symbol:
KRT14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P02533
User NOte:
Optimal dilutions for each application to be determined by the researcher.