ITGAX Antibody

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ProSci
Product Code: PSI-15-864
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-15-864-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ITGAX antibody (15-864) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat lung using ITGAX antibody (15-864) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human tonsil using ITGAX antibody (15-864) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using ITGAX antibody (15-864) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using ITGAX antibody (15-864) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using ITGAX antibody (15-864) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat lung using ITGAX antibody (15-864) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using ITGAX antibody (15-864) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using ITGAX antibody (15-864) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using ITGAX antibody (15-864) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
Integrin alpha-X, CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150, CD11c, ITGAX, CD11C
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-350 of human ITGAX (NP_000878.2).
NCBI Gene ID #:
3687
NCBI Official Name:
integrin, alpha X (complement component 3 receptor 4 subunit)
NCBI Official Symbol:
ITGAX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 150kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction, Stem Cell
Swissprot #:
P20702
User NOte:
Optimal dilutions for each application to be determined by the researcher.