CES2 Antibody

ProSci
Product Code: PSI-15-900
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-900-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CES2 antibody (15-900) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human colon using CES2 antibody (15-900) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using CES2 antibody (15-900). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CES2 antibody (15-900) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon using CES2 antibody (15-900) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using CES2 antibody (15-900). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CES2, CE-2, iCE, PCE-2, CES2A1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 310-410 of human CES2 (NP_003860.3).
NCBI Gene ID #:
8824
NCBI Official Name:
carboxylesterase 2
NCBI Official Symbol:
CES2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 67kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O00748
User NOte:
Optimal dilutions for each application to be determined by the researcher.