Acetyl-Histone H4-K5 pAb

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ProSci
Product Code: PSI-15-941
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-15-941-50uL50uL£505.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (15-941) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat brain using H4K5AC antibody (15-941) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human tonsil using H4K5AC antibody (15-941) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using H4K5AC antibody (15-941) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using H4K5AC antibody (15-941) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (15-941) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using H4K5AC antibody (15-941) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using H4K5AC antibody (15-941) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using H4K5AC antibody (15-941) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using H4K5AC antibody (15-941) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

ChIP: 1:20 - 1:100
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1).
Modifications:
Acetylated
NCBI Gene ID #:
8370
NCBI Official Name:
histone cluster 1, H4a
NCBI Official Symbol:
H4K5AC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 11kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P62805
User NOte:
Optimal dilutions for each application to be determined by the researcher.