EPRS Antibody

ProSci
Product Code: PSI-15-945
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-945-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EPRS antibody (15-945) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using EPRS antibody (15-945) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Further Information

Additional Names:
EPRS, DKFZp313B047, EARS, PARS, PIG32, QARS, QPRS, GLUPRORS
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100
Background:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human EPRS (NP_004437.2).
NCBI Gene ID #:
2058
NCBI Official Name:
glutamyl-prolyl-tRNA synthetase
NCBI Official Symbol:
EPRS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 190kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P07814
User NOte:
Optimal dilutions for each application to be determined by the researcher.