ATP1A4 Antibody

ProSci
Product Code: PSI-15-950
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-950-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATP1A4 antibody (15-950) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1S.

Western blot analysis of extracts of various cell lines, using ATP1A4 antibody (15-950) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1S.

Further Information

Additional Names:
ATP1A1, ATP1AL2, sodium/potassium-transporting ATPase subunit alpha-4, ATPase, Na+/K+ transporting, alpha 4 polypeptide, ATPase, Na+/K+ transporting, alpha polypeptide-like 2, Na(+)/K(+) ATPase alpha-4 subunit, Na+/K+ ATPase 4, Na+/K+ ATPase, alpha-D polypeptide, Na,K-ATPase subunit alpha-C, sodium pump 4, sodium pump subunit alpha-4, sodium-potassium ATPase catalytic subunit alpha-4, sodium/potassium-transporting ATPase alpha-4 chain
Application Note:
WB: 1:200 - 1:2000
Background:
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human ATP1A4 (NP_653300.2).
NCBI Gene ID #:
480
NCBI Official Name:
ATPase Na+/K+ transporting subunit alpha 4
NCBI Official Symbol:
ATP1A4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13733
User NOte:
Optimal dilutions for each application to be determined by the researcher.