ATP5C1 Antibody

ProSci
Product Code: PSI-15-951
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-951-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ATP5C1 antibody (15-951) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5S.
2 / 4
Immunofluorescence analysis of C6 cells using ATP5C1 Polyclonal Antibody (15-951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of L929 cells using ATP5C1 Polyclonal Antibody (15-951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of U-2 OS cells using ATP5C1 Polyclonal Antibody (15-951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ATP5C1 antibody (15-951) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5S.
Immunofluorescence analysis of C6 cells using ATP5C1 Polyclonal Antibody (15-951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using ATP5C1 Polyclonal Antibody (15-951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ATP5C1 Polyclonal Antibody (15-951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ATP5C1, ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1, ATP5C, ATP5CL1, ATP synthase gamma chain, mitochondrial, ATP synthase subunit gamma, mitochondrial, F-ATPase gamma subunit, mitochondrial ATP synthase, gamma subunit 1
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-298 of human ATP5C1 (NP_001001973.1).
NCBI Gene ID #:
509
NCBI Official Name:
ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
NCBI Official Symbol:
ATP5C1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P36542
User NOte:
Optimal dilutions for each application to be determined by the researcher.