BTF3 Antibody

ProSci
Product Code: PSI-15-952
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-952-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using BTF3 antibody (15-952) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10S.

Western blot analysis of extracts of various cell lines, using BTF3 antibody (15-952) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10S.

Further Information

Additional Names:
BTF3, NACB, BTF3a, BTF3b, BETA-NAC
Application Note:
WB: 1:200 - 1:2000
Background:
This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human BTF3 (NP_001198.2).
NCBI Gene ID #:
689
NCBI Official Name:
basic transcription factor 3
NCBI Official Symbol:
BTF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 22kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
P20290
User NOte:
Optimal dilutions for each application to be determined by the researcher.