MYH2 Antibody

ProSci
Product Code: PSI-15-967
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-15-967-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MYH2 antibody (15-967) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 180s.
2 / 2
Immunofluorescence analysis of mouse skeletal muscle using MYH2 Polyclonal Antibody (15-967) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MYH2 antibody (15-967) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 180s.
Immunofluorescence analysis of mouse skeletal muscle using MYH2 Polyclonal Antibody (15-967) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
IBM3A, MYHSA2, MYHas8, MYPOP, MyHC-2A, MyHC-IIa, MYH2, myosin-2, fast 2a myosin heavy chain, inclusion body myopathy 3, autosomal dominant, myosin heavy chain 2a, myosin heavy chain IIa, myosin heavy chain, skeletal muscle, adult 2, myosin, heavy chain 2, skeletal muscle, adult, myosin, heavy polypeptide 2, skeletal muscle, adult, type IIA myosin heavy chain
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human MYH2 (NP_001093582.1).
NCBI Gene ID #:
4620
NCBI Official Name:
myosin heavy chain 2
NCBI Official Symbol:
MYH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 223kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9UKX2
User NOte:
Optimal dilutions for each application to be determined by the researcher.