PRG4 Antibody

ProSci
Product Code: PSI-16-021
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-021-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 1
Western blot analysis of extracts of LO2 cells, using PRG4 antibody (16-021) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Western blot analysis of extracts of LO2 cells, using PRG4 antibody (16-021) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Further Information

Additional Names:
PRG4, proteoglycan 4, CACP, FLJ32635, HAPO, JCAP, MSF, SZP, bG174L6.2, Jacobs camptodactyly-arthropathy-pericarditis syndrome, articular superficial zone protein, bG174L6.2 (MSF: megakaryocyte stimulating factor ), camptodactyly, arthropathy, coxa vara,
Application Note:
WB: 1:200 - 1:2000
Background:
The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1264-1363 of human PRG4 (NP_001121180.2).
NCBI Gene ID #:
10216
NCBI Official Name:
proteoglycan 4
NCBI Official Symbol:
PRG4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 150kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q92954
User NOte:
Optimal dilutions for each application to be determined by the researcher.