AFG3L2 Antibody

ProSci
Product Code: PSI-16-029
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-029-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using AFG3L2 antibody (16-029) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat brain using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using AFG3L2 antibody (16-029) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
AFG3L2, FLJ25993, SCA28, SPAX5
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 538-797 of human AFG3L2 (NP_006787.2).
NCBI Gene ID #:
10939
NCBI Official Name:
AFG3-like AAA ATPase 2
NCBI Official Symbol:
AFG3L2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 89kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q9Y4W6
User NOte:
Optimal dilutions for each application to be determined by the researcher.