MICAL1 Antibody

ProSci
Product Code: PSI-16-080
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-080-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MICAL1 antibody (16-080) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using MICAL1 antibody (16-080) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Further Information

Additional Names:
MICAL, MICAL-1, NICAL, [F-actin]-monooxygenase MICAL1, NEDD9-interacting protein with calponin homology and LIM domains, [F-actin]-methionine sulfoxide oxidase MICAL1, molecule interacting with CasL protein 1, protein-methionine sulfoxide oxidase MICAL1
Application Note:
WB: 1:200 - 1:2000
Background:
This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 718-1067 of human MICAL1 (NP_073602.3).
NCBI Gene ID #:
64780
NCBI Official Name:
microtubule associated monooxygenase, calponin and LIM domain containing 1
NCBI Official Symbol:
MICAL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 132kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
Q8TDZ2
User NOte:
Optimal dilutions for each application to be determined by the researcher.