RAPH1 Antibody

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ProSci
Product Code: PSI-16-081
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-081-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RAPH1 antibody (16-081) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Western blot analysis of extracts of various cell lines, using RAPH1 antibody (16-081) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Documents

Further Information

Additional Names:
ALS2CR18, ALS2CR9, LPD, PREL-2, PREL2, RMO1, RalGDS/AF-6, ras-associated and pleckstrin homology domains-containing protein 1, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 18, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 9, lamellipodin, proline-rich EVH1 ligand 2
Application Note:
WB: 1:200 - 1:2000
Background:
This gene encodes a protein that belongs to the Mig10/Rap1-interacting adaptor molecule/Lamellipodin family of adapter proteins, which function in cell migration. Members of this family contain pleckstrin-homology domains, Ras-association domains, and proline-rich C-termini. The protein encoded by this gene regulates actin dynamics through interaction with Ena/Vasodilator proteins as well as direct binding to filamentous actin to regulate actin network assembly. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RAPH1 (NP_998754.1).
NCBI Gene ID #:
65059
NCBI Official Name:
Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
NCBI Official Symbol:
RAPH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 200kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q70E73
User NOte:
Optimal dilutions for each application to be determined by the researcher.