DRD3 Antibody

ProSci
Product Code: PSI-16-111
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-111-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse brain, using DRD3 antibody (16-111) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat brain using DRD3 Antibody (16-111) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using DRD3 Antibody (16-111) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using DRD3 Antibody (16-111) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using DRD3 antibody (16-111). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse brain, using DRD3 antibody (16-111) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using DRD3 Antibody (16-111) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DRD3 Antibody (16-111) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using DRD3 Antibody (16-111) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DRD3 antibody (16-111). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DRD3, D3 dopamine receptor, D3DR, Dopamine 3 receptor, Dopamine receptor D3, D3nf, Essential tremor 1, ETM1, FET1, D(3) dopamine receptor, Dopamine D3 receptor
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the D3 subtype of the five (D1-D5) dopamine receptors. The activity of the D3 subtype receptor is mediated by G proteins which inhibit adenylyl cyclase. This receptor is localized to the limbic areas of the brain, which are associated with cognitive, emotional, and endocrine functions. Genetic variation in this gene may be associated with susceptibility to hereditary essential tremor 1. Alternative splicing of this gene results in transcript variants encoding different isoforms, although some variants may be subject to nonsense-mediated decay (NMD).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 213-329 of human DRD3 (NP_000787.2).
NCBI Gene ID #:
1814
NCBI Official Name:
dopamine receptor D3
NCBI Official Symbol:
DRD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P35462
User NOte:
Optimal dilutions for each application to be determined by the researcher.