POLRMT Antibody

ProSci
Product Code: PSI-16-132
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-132-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using POLRMT antibody (16-132) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug POLRMT antibody (16-132). Western blot was performed from the immunoprecipitate using POLRMT antibody (16-132) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using POLRMT antibody (16-132) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug POLRMT antibody (16-132). Western blot was performed from the immunoprecipitate using POLRMT antibody (16-132) at a dilition of 1:1000.

Further Information

Additional Names:
POLRMT, polymerase (RNA) mitochondrial (DNA directed), APOLMT, MTRNAP, MTRPOL, h-mtRPOL, DNA-directed RNA polymerase, mitochondrial
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Background:
This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3).
NCBI Gene ID #:
5442
NCBI Official Name:
polymerase (RNA) mitochondrial (DNA directed)
NCBI Official Symbol:
POLRMT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 139kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O00411
User NOte:
Optimal dilutions for each application to be determined by the researcher.