TRAF7 Antibody

ProSci
Product Code: PSI-16-134
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-134-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TRAF7 antibody (16-134) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.

Western blot analysis of extracts of various cell lines, using TRAF7 antibody (16-134) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.

Further Information

Additional Names:
CAFDADD, RFWD1, RNF119, E3 ubiquitin-protein ligase TRAF7, RING finger and WD repeat-containing protein 1, RING finger protein 119, RING-type E3 ubiquitin transferase TRAF7, TNF receptor-associated factor 7, E3 ubiquitin protein ligase, ring finger and WD repeat domain 1
Application Note:
WB: 1:500 - 1:2000
Background:
Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-400 of human TRAF7 (NP_115647.2).
NCBI Gene ID #:
84231
NCBI Official Name:
TNF receptor associated factor 7
NCBI Official Symbol:
TRAF7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Chemokines & Cytokines, Immunology, Innate Immunity
Swissprot #:
Q6Q0C0
User NOte:
Optimal dilutions for each application to be determined by the researcher.