Acetyl-Histone H2B-K12 pAb

Contact us
ProSci
Product Code: PSI-16-140
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-140-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 7
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K12 antibody (16-140) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours.C2C12 cells were treated by TSA (1 uM) at 37°C for 18 hours.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 7
Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens).
3 / 7
Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens).
5 / 7
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
6 / 7
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
7 / 7
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K12 antibody (16-140) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours.C2C12 cells were treated by TSA (1 uM) at 37°C for 18 hours.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 antibody (16-140) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
H2B, H2BQ, GL105, H2B.1, H2BFQ, H2BGL105
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1).
Modifications:
Acetylated
NCBI Gene ID #:
8349
NCBI Official Name:
histone cluster 2, H2be
NCBI Official Symbol:
H2B K12ac
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q16778
User NOte:
Optimal dilutions for each application to be determined by the researcher.