Acetyl-Histone H2A-K5 pAb

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ProSci
Product Code: PSI-16-141
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-16-141-50uL50uL£505.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Immunohistochemistry of paraffin-embedded human breast using H2AK5ac antibody (16-141) at dilution of 1:100 (40x lens).
2 / 4
Immunohistochemistry of paraffin-embedded mouse liver using H2AK5ac antibody (16-141) at dilution of 1:100 (40x lens).
3 / 4
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (16-141) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody (16-141) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded human breast using H2AK5ac antibody (16-141) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using H2AK5ac antibody (16-141) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (16-141) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody (16-141) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Histone H2A type 1, H2A1, Histone H2A/p, HIST1H2AG, H2AFP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).
Modifications:
Acetylated
NCBI Gene ID #:
8329
NCBI Official Name:
Histone H2A type 1
NCBI Official Symbol:
H2A K5ac
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P0C0S8
User NOte:
Optimal dilutions for each application to be determined by the researcher.