Acetyl-Histone H2B-K5 pAb

Contact us
ProSci
Product Code: PSI-16-142
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-142-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K5 antibody (16-142) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 4
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 antibody (16-142) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 antibody (16-142) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 antibody (16-142) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K5 antibody (16-142) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 antibody (16-142) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 antibody (16-142) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 antibody (16-142) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.

Documents

Further Information

Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:100

ChIP: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic acetylated peptide corresponding to residues surrounding K5 of human Histone H2B
Modifications:
Acetylated
NCBI Official Symbol:
H2B K5ac
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher.