Acetyl-Histone H2B-K15 pAb

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ProSci
Product Code: PSI-16-143
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-16-143-50uL50uL£505.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K15 antibody (16-143) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K15 antibody (16-143) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% BSA.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using H2B K15ac antibody (16-143) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K15 antibody (16-143) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining.

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Further Information

Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
AA synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B
Modifications:
Acetylated
NCBI Official Symbol:
H2B K15ac
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Affinity purification
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher.