CDK9 Antibody

ProSci
Product Code: PSI-16-157
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-157-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CDK9 antibody (16-157) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of U2OS cells using CDK9 antibody (16-157). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CDK9 antibody (16-157). Western blot was performed from the immunoprecipitate using CDK9 antibody (16-157) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using CDK9 antibody (16-157) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using CDK9 antibody (16-157). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CDK9 antibody (16-157). Western blot was performed from the immunoprecipitate using CDK9 antibody (16-157) at a dilition of 1:1000.

Further Information

Additional Names:
CDK9, RP11-228B15.5, C-2k, CDC2L4, PITALRE, TAK, CTK1
Application Note:
WB: 1:1000 - 1:3000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 153-372 of human CDK9 (NP_001252.1).
NCBI Gene ID #:
1025
NCBI Official Name:
cyclin-dependent kinase 9
NCBI Official Symbol:
CDK9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P50750
User NOte:
Optimal dilutions for each application to be determined by the researcher.