TSPO Antibody

ProSci
Product Code: PSI-16-159
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-159-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of mouse testis, using TSPO antibody (16-159) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 2
Immunofluorescence analysis of L929 cells using TSPO Polyclonal Antibody (16-159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse testis, using TSPO antibody (16-159) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of L929 cells using TSPO Polyclonal Antibody (16-159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
TSPO, translocator protein (18 kDa), BZRP, DBI, IBP, MBR, PBR, PKBS, PTBR, mDRC, pk18, OTTHUMP00000028891, benzodiazapine receptor (peripheral), benzodiazepine peripheral binding site, mitochondrial benzodiazepine receptor, peripheral benzodiazepine receptor, peripheral-type benzodiazepine receptor
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-50 of human TSPO (NP_000705.2).
NCBI Gene ID #:
706
NCBI Official Name:
translocator protein (18kDa)
NCBI Official Symbol:
TSPO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 19kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P30536
User NOte:
Optimal dilutions for each application to be determined by the researcher.