CHRM2 Antibody

ProSci
Product Code: PSI-16-170
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-170-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CHRM2 antibody (16-170) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of MCF-7 cells using CHRM2 antibody (16-170). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CHRM2 antibody (16-170) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of MCF-7 cells using CHRM2 antibody (16-170). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
7TM receptor, cholinergic receptor, muscarinic 2, CHRM2, FLJ43243, HM2, MGC120006, MGC120007, muscarinic acetylcholine receptor M2, muscarinic M2 receptor, OTTHUMP00000208393, OTTHUMP00000208394, OTTHUMP00000208395, OTTHUMP00000208396
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 208-388 of human CHRM2 (NP_001006629.1).
NCBI Gene ID #:
1129
NCBI Official Name:
cholinergic receptor, muscarinic 2
NCBI Official Symbol:
CHRM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P08172
User NOte:
Optimal dilutions for each application to be determined by the researcher.