SIN3A Antibody

ProSci
Product Code: PSI-16-224
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-224-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using SIN3A antibody (16-224) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of U2OS cells using SIN3A antibody (16-224). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SIN3A antibody (16-224). Western blot was performed from the immunoprecipitate using SIN3A antibody (16-224) at a dilition of 1:500.

Western blot analysis of extracts of various cell lines, using SIN3A antibody (16-224) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using SIN3A antibody (16-224). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SIN3A antibody (16-224). Western blot was performed from the immunoprecipitate using SIN3A antibody (16-224) at a dilition of 1:500.

Further Information

Additional Names:
SIN3A, DKFZP434K2235, FLJ90319, KIAA0700,
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100

ChIP: 1:20 - 1:100
Background:
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SIN3A (NP_056292.1).
NCBI Gene ID #:
25942
NCBI Official Name:
SIN3 transcription regulator family member A
NCBI Official Symbol:
SIN3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 165kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience
Swissprot #:
Q96ST3
User NOte:
Optimal dilutions for each application to be determined by the researcher.