ProSci

ZNRF1 Antibody

Product Code:
 
PSI-16-322
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using ZNRF1 antibody (16-322) at 1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of various cell lines, using ZNRF1 antibody (16-322) at 1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

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PSI-16-322-50uL50uL£433.00
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Further Information

Additional Names:
Znrf1, zinc and ring finger 1, B830022L21Rik, MGC101991, Rnf42, Zrfp1, nin283, E3 ubiquitin-protein ligase ZNRF1, nerve injury-induced gene 283 protein, peripheral nerve injury protein nin283, ring finger protein 42, zinc ring finger protein 1, zinc/RING
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ZNRF1 (NP_115644.1).
NCBI Gene ID #:
84937
NCBI Official Name:
zinc and ring finger 1, E3 ubiquitin protein ligase
NCBI Official Symbol:
ZNRF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 24kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q8ND25
User NOte:
Optimal dilutions for each application to be determined by the researcher.