ZNRF3 Antibody

ProSci
Product Code: PSI-16-383
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-383-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ZNRF3 antibody (16-383) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat lung using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ZNRF3 antibody (16-383) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using ZNRF3 antibody (16-383) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
BK747E2.3, RNF203, E3 ubiquitin-protein ligase ZNRF3, CTA-292E10.6, RING finger protein 203, RING-type E3 ubiquitin transferase ZNRF3, novel C3HC4 type Zinc finger (ring finger), zinc/RING finger protein 3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ZNRF3 (NP_115549.2).
NCBI Gene ID #:
84133
NCBI Official Name:
zinc and ring finger 3
NCBI Official Symbol:
ZNRF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 120kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9ULT6
User NOte:
Optimal dilutions for each application to be determined by the researcher.