RARB Antibody

ProSci
Product Code: PSI-16-384
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-384-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RARB Antibody (16-384) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using RARB antibody (16-384) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using RARB antibody (16-384) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RARB Antibody (16-384) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using RARB antibody (16-384) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using RARB antibody (16-384) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
RARB, HAP, NR1B2, RRB2, MCOPS12
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. Alternate promoter usage and differential splicing result in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human RARB
NCBI Gene ID #:
5915
NCBI Official Name:
retinoic acid receptor, beta
NCBI Official Symbol:
RARB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Transcription
Swissprot #:
P10826
User NOte:
Optimal dilutions for each application to be determined by the researcher.