USO1 Antibody

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ProSci
Product Code: PSI-16-417
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-417-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using USO1 antibody (16-417) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunofluorescence analysis of HeLa cells using USO1 antibody (16-417) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.
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Confocal immunofluorescence analysis of Hela cells using USO1 antibody (16-417) at dilution of 1:200. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using USO1 antibody (16-417) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using USO1 antibody (16-417) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using USO1 antibody (16-417) at dilution of 1:200. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
vesicle docking protein p115, transcytosis associated protein, USO1 homolog, vesicle docking protein, vesicle docking protein (yeast), USO1, transcytosis-associated protein, TAP, P115, VDP p115, VDP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 663-962 of human USO1 (NP_003706.2).
NCBI Gene ID #:
8615
NCBI Official Name:
USO1 vesicle transport factor
NCBI Official Symbol:
USO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 108kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O60763
User NOte:
Optimal dilutions for each application to be determined by the researcher.