FABP2 Antibody

ProSci
Product Code: PSI-16-492
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-492-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using FABP2 antibody (16-492) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunofluorescence analysis of HeLa cells using FABP2 antibody (16-492). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using FABP2 antibody (16-492) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using FABP2 antibody (16-492). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
FABP2, fatty acid binding protein 2, intestinal, FABPI, I-FABP, MGC133132, Fatty acid-binding protein, intestinal fatty acid binding protein 2
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human FABP2 (NP_000125.2).
NCBI Gene ID #:
2169
NCBI Official Name:
fatty acid binding protein 2, intestinal
NCBI Official Symbol:
FABP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 15kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P12104
User NOte:
Optimal dilutions for each application to be determined by the researcher.