NTN1 Antibody

ProSci
Product Code: PSI-16-506
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-506-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NTN1 antibody (16-506) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
2 / 2
Immunofluorescence analysis of U-251MG cells using NTN1 antibody (16-506) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NTN1 antibody (16-506) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
Immunofluorescence analysis of U-251MG cells using NTN1 antibody (16-506) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
netrin 1, mouse, homolog of, Netrin-1, NTN1L, NTN1, HGNC:8029, netrin 1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human NTN1 (NP_004813.2).
NCBI Gene ID #:
9423
NCBI Official Name:
netrin 1
NCBI Official Symbol:
NTN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
O95631
User NOte:
Optimal dilutions for each application to be determined by the researcher.