LPL Antibody

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ProSci
Product Code: PSI-16-512
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-512-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using LPL antibody (16-512) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunofluorescence analysis of C6 cells using LPL antibody (16-512) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using LPL antibody (16-512) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of U-2OS cells using LPL antibody (16-512) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using LPL antibody (16-512) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of C6 cells using LPL antibody (16-512) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using LPL antibody (16-512) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2OS cells using LPL antibody (16-512) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
Lipoprotein lipase, LPL, LPL, LIPD
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 124-475 of human LPL (NP_000228.1).
NCBI Gene ID #:
4023
NCBI Official Name:
Lipoprotein lipase
NCBI Official Symbol:
LPL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Obesity, Signal Transduction, Stem Cell
Swissprot #:
P06858
User NOte:
Optimal dilutions for each application to be determined by the researcher.