DDX6 Antibody

ProSci
Product Code: PSI-16-517
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-517-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DDX6 antibody (16-517) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat brain using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human mammary cancer using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of L929 cells using DDX6 Polyclonal Antibody (16-517) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using DDX6 antibody (16-517) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using DDX6 antibody (16-517) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using DDX6 Polyclonal Antibody (16-517) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DDX6, P54, RCK, HLR2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human DDX6 (NP_001244120.1).
NCBI Gene ID #:
1656
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box helicase 6
NCBI Official Symbol:
DDX6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 54kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P26196
User NOte:
Optimal dilutions for each application to be determined by the researcher.